The research aimed to study H2SO4 during hydrolysis and the role of molasses in the yield of fermentation. Experiment was designed in CRD (Completely Randomized Design)with six treatments : (1) 3 hours of H2SO4 hydrolisis adding with 10% of molasses, (2) 4 hours of H2SO4 hydrolisis adding with 10% of mollases, (3) 5 hours of H2SO4 hydrolisis adding with 10% of mollases, (4) 3 hours H2SO4 hydrolisis adding with 15% of mollases, (5) 4 hours of H2SO4 hydrolisis adding with 15% of mollases, and (6) 5 hours H2SO4 hydrolisis adding with 15% of mollases. Result showed that the longer of H2SO4 hydrolisis and the higher of mollases concentration produced higher percentage of sugar. Hydrolysis in 5 hours and mollases concentration of 15% gave the best result on sugar production. However the highest concentration of ethanol was obtained in 3 hours hydrolysis with mollasees concentration of 15%.