Anti-emetic or nausea drug is a drug used to treat nausea and vomiting. The cause nausea and vomiting have a variety such as: food allergy, infection of the stomach or food poisoning, leaking of stomach contents (food or liquid) above which is also called gastroesophageal reflux or GERD. The purpose of this study was to determine the effect of the compound class of essential oils Ginger (Zingiber officinale) on Indonesian medicinal plants against smooth muscle contraction induced ileum agonist acetylcholine. The method used is in silico and in vitro, is to perform molecular docking using AutoDockTools applications and experiments on smooth muscle of guinea pig ileum test animal (Cavia cobaya) using the tool organ bath. Analysis of this form of comparative molecular docking of ligand docking original score (Tiotropium), the test compound (zingiberene) and a comparator drug (Atropine Sulfate). Ginger essential oils given at a dose of 1 ppm and 1,25 ppm (part per million), while agonist given the rate series 10-8 - 10-2 M. In vitro tests have also examined the nature reversibility at muscarinic acetylcholine receptors (ACh M3). The results of this study indicate that ginger essential oil dose of 1 ppm and 1,25 ppm is able to inhibit smooth muscle contraction response of isolated guinea pig ileum induced by acetylcholine concentration series. This was shown by a shift in the response curve isolated ileum smooth muscle contraction in the right direction with a dose-dependent pattern. Docking visualization results showed that the test compound and the native ligand attached to the same residues, namely tyrosine to-529. Ginger also has binding energy that is stronger than the Atropine as a comparison compound. From the results of docking marker compounds and experiments on isolated ileum smooth muscle of essential oils of ginger as a potential anti-emetic agent.