Hemodialysis is a procedure in which blood is removed from the patient's body and circulates in a machine outside the body called dialyzer. The frequency of hemodialysis action varies depending on the number of kidney function remaining, the average patient underwent two or three times per week. Creatinine is a chemical in the body that was one objective of Hemodialysis. There is still a lack of research that addresses the frequency of hemodialysis related to creatinine levels, so these studies are necessary to evaluate the effectiveness of hemodialysis frequency in a week against a decrease in creatinine level of patients with chronic renal failure. Methods: This study was cross sectional approach. Those samples were taken with purpossive sampling technique. The instruments used are the property of medical records of patients undergoing hemodialysis. Analysis of the data used is observational analytic. Results: In the group of hemodialysis patients 2 times per week got 3 person (5,8%) had serum creatinine level <3 mg / dL and 15 person (28,8%) had serum creatinine level >3 mg / dL, while in the group of patients hemodialysis 3 times per week obtained 8 person (15,4%) had serum creatinine level <3 mg / dL and 26 person (50%) had serum creatinine level >3 mg / dL. The results of chi-square analysis showed the P value was 0,564. which means that the value of p > 0,05. Conclusion: There is no influenced on the frequency of hemodialysis blood creatinine levels decrease in patients with chronic renal failure.