The rapid development of technology in recent years led to the emergence of various types of Internet-based businesses, one of which is Tokopedia as a marketplace that brings together sellers and buyers online. The title of this research is "influence of Perceived Usefulness, Perceived Ease of Use and E-Service Quality of E-Customer Satisfaction on Tokopedia Online Shop Customers" aimed at analyzing how the acceptance of technology use and the quality of electronic services that are felt to influence the satisfaction of doing transactions on the Tokopedia website. The subjects in this study are customers who have made transactions at least 2 times in the past year on the Tokopedia site. This research was conducted with a total sample of 125 respondents using a non-probability sampling technique with a purposive sampling method and using a survey method by distributing questionnaires using Google Forms. The analytical tool for analyzing data is the SPSS (Statistical Package for Social Sciences). The result of classical assumption test show the regression model is proper. Based on the analysis that has been done, the results show that Perceived Usefulness, Perceived Ease of Use and E-Service Quality significantly influence E-Customer Satisfaction.