This study aims to determine the effect of organizational culture on performance with job satisfaction and organizational commitment as an intervening variable. The object of this research is Muhammadiyah University of Yogyakarta. The subjects of this study were Muhammadiyah Yogyakarta University employees using convenience sampling, and the data analysis technique used was Path Analysis. Based on the results of simple regression test analysis and multiple regression obtained organizational culture results have a significant effect on job satisfaction. This is evidenced by a significant value of 0,000 < 0,05. Organizational culture has a significant effect on organizational commitment. This is evidenced by a significant value of 0,000 < 0,05. Then job satisfaction has a significant effect on employee performance. This is evidenced by a significant value of 0.007 < 0.05. Organizational commitment has a significant effect on employee performance. This is evidenced by a significant value of 0,000 <0,05. Organizational culture has a significant effect on employee performance. This is evidenced by a significant value of 0,000 < 0,05. Then the path analysis was carried out and the direct influence of organizational culture on employee performance was 0.189. The value of indirect influence through job satisfaction is 1.91 which is greater than the value of direct influence. Then the value of indirect influence through organizational commitment is 1.99 which is greater than the value of direct influence. Then a sobel test was conducted to strengthen the results of path analysis, and t value was calculated through job satisfaction of 2.195> 1.669 (t table) and through organizational commitment of 1.686> 1.669 (t table), so it can be concluded that job satisfaction and organizational commitment are variables intervening from the influence of organizational culture on employee performance.employee performance.