There are a lot of incident of infection diseases on skin and mouth that caused by fungi in Indonesia. Candida Albican is one type of fungus that can cause infection. From the previous research, it was known that the parts of Pisang Ambon plant has high content of saponin compounds, which are allegedly active as antifungal agents. This study is aimed to know the antifungal activity of the ethanol extract, n-hexane fraction and the fraction of ethyl acetate extract ethanolic from the stem of Pisang Ambon Candida albicans as In Vitro. The extraction process of Pisang Ambon’s stem was done by maceration method. The first step was analyze the content of saponin compounds in qualitative with Forth test methods. TLC test was done to identify any compounds that was contained in each fraction. The process of fractionation done with liquidliquid extraction using ethanol solvent as a polar fraction, n-hexane solvent as non-polar fraction, and ethyl acetate solvent as semi-polar fraction. The experiment of antifungal activity was done with liquid dilution method. The concentration of each fraction were 6,25 mg/ml, 12,5 mg/ml, 25 mg/ml, 50 mg/ml, and 100 mg/ml w/v. Each of the concentration was measured with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) to know the exact concentration that could inhibit or kill Candida albicans. The result of compound identification showed that the extraction from Pisang Ambon’s stem had the content of saponin compound with the onset of the foam was great in the Forth test. The result of TLC test was showed that there was saponin compound in n-hexane fraction and ethyl acetat fraction. The result of antifungal activity research on Pisang Ambon’ stem against Candida albicans was obtained the value of MIC and MBC were the same on each fraction, with namely in sequence at a concentration of 6,25 mg/ml and 100 mg/ml w/v.